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Plant Pest Diagnostics Center

Tongyan Tian

Dr. Tongyan Tian

Senior Plant Pathologist - Specialist

Plant Pest Diagnostics Branch, California Department of Food & Agriculture
3294 Meadowview Road, Sacramento, CA 95832-1448, U.S.A.
916-262-1127 office, 916-262-1190 fax, ttian@cdfa.ca.gov

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Curriculum vitae

Education:

  • 1982, B.S., Shenyang Agricultural College
  • 1991, Ph.D., University of California, Davis

Positions held:

Senior Plant Pathologist (Diagnostician), CDFA-PPDB

  • Responsible for plant virus diagnostics. 

1997-2001: SRA, Dept. of Plant Pathology, UC Davis

  • Study of Lettuce infectious yellows virus (LIYV, in the family Closteroviridae) insect transmission.
  • LIYV ultra structure: Demonstrated the polar virion structure using immuno-gold labeling and transmission electron microscopy.

1992-1996: Visiting Postdoctoral Researcher, Dept. of Plant Pathology, UC Davis

  • Evaluation of resistance of transgenic lettuce and Nicotiana benthamiana plants to LIYV.
  • Identification of the viral components in carrot motley dwarf complex.
  • Evaluation of the use of a baculovirus, Bombyx mori nuclear polyhedrosis virus (baculovirus), for protein expressions of the capsid protein, P19 and readthrough of beet western yellows virus (luteovirus), the capsid protein of bean golden mosaic virus (geminivirus), and non-capsid protein of maize stripe virus (tenuivirus).

1992 Feb.-1992 Aug.: Postdoctoral Researcher, Dept. of Entomology, UC Davis

  • Participated in the study of Bombyx mori nuclear polyhedrosis virus protein expression system.
  • Participated in analysis of a soluble epoxide hydrolase from human liver.

Publications

  1. Medina V., Sudarshana, M.R., Tian, T., Ralston, K.S., Yeh, H.H., Falk, B.W. 2005. The Lettuce infectious yellows virus (LIYV)-encoded P26 is associated with plasmalemma deposits within LIYV-infected cells.  Virology. 2005 Mar 15;333(2):367-73.
  2. Nagata. T, Alves, D.M., Inoue-Nagata, A.K., Tian, T.Y., Kitajima, E.W., Cardoso, J.E., de Avila, A.C. 2005. A novel melon flexivirus transmitted by whitefly.   Arch Virol. 2005 Feb;150(2):379-87.
  3. Ng JC, Tian, T., Falk BW. 2004. Quantitative parameters determining whitefly (Bemisia tabaci) transmission of Lettuce infectious yellows virus and an engineered defective RNA.   J Gen Virol. 2004 Sep;85(Pt 9):2697-707.
  4. Medina V., Rodrigo G., Tian T., Juarez M., Dolja V.V., Achon M.A., and Falk B.W. 2003. Comparative cytopathology of Crinivirus infections in different plant hosts.  Annals of Applied Biology.  143 (1), 99 - 110.
  5. Rubio, L, Tian, T. Yeh, H-H., Livieratos, Y, and Falk, B. W. 2002. De novo generation of Lettuce infectious yellows virus defective RNAs in protoplasts.  Molecular Plant Pathology.  3(5) 321-327.
  6. Yeh, H-H., Tian, T., Medina, V., and Falk, B. W. 2001. Green fluorescent protein expression from recombinant Lettuce infectious yellows virus-defective RNAs originating from RNA 2.  Virology 289, 54-62.
  7. Yeh, H-H., Tian, T., Rubio, L., Crawford, B., and Falk, B. W. 2000. Asynchronous accumulation of lettuce infectious yellows virus RNAs 1 and 2 and identification of an RNA 1 trans enhancer of RNA 2 accumulation.  J. Virology. 74: 5762-5768.
  8. Rubio, L., Yeh, H-H., Tian, T., Falk, B.W. 2000. A heterogeneous population of defective RNAs is associated with lettuce infectious yellows virus (LIYV).  Virology 271, 205-212.
  9. Kao, J., Jia, L., Tian, T., Rubio, L., Falk, B. W. 2000. First report of cucurbit yellow stunting disorder virus (Genus Crinivirus) in North America.  Plant Disease 84: 101.
  10. Rodrigo, G., Wierzchos, J., Tian, T., and Falk, B. W. 1999. In situ localization of lettuce infectious yellows and maize stripe viruses by immunogold labeling and laser scanning confocal microscopy in reflected signal detection mode.  ACTA Microscopica 8: 1-9
  11. Tian, T.; Rubio, L., Yeh, H-H, Falk, B.W. 1999. Lettuce infectious yellows virus: in vitro acquisition analysis using purified virions and the whitefly Bemisia tabaci.  J. Gen. Virol. 80: 1111-1117.
  12. Falk, B. W., Tian, T., and Yeh, H.-H. 1999. Luteovirus-associated viruses and sub-viral RNAs. Curr. Top. Microbiol. Immunol.  239:159-75.
  13. Falk, B. W., and Tian, T. 1999. Transcapsidation interactions and dependent aphid transmission among luteoviruses, and luteovirus-associated RNAs.  Invited Chapter.  The Luteoviridae, Smith, H. G., Barker, H., and Jones, P., eds.  CAB International. pp 125-134.
  14. Watson, M. T., Tian, T., Estabrook, E., and Falk, B. W. 1998. A small RNA identified as a component of California carrot motley dwarf resembles the beet western yellows luteovirus ST9-associated RNA.  Phytopathology 88:164 – 170.
  15. Medina, V., Tian, T., Wierzchos, J., and Falk, B. W. 1998. Specific inclusion bodies are associated with replication of lettuce infectious yellows virus (LIYV) RNAs in Nicotiana benthamiana protoplasts.  J. Gen. Virol.  78: 2325 – 2329.
  16. Tian, T., Klaassen, V. A., Soong, J., Wisler, G., Duffus, J. E., and Falk, B. W. 1996. Generation of cDNAs specific to lettuce infectious yellows closterovirus and other whitefly-transmitted viruses by using RT-PCR and degenerate oligonucleotide primers corresponding to the closterovirus gene encoding the heat shock protein 70 homolog.  Phytopathology 86: 1167-1173.
  17. Klaassen, V.A., Boeshore, M.L., Koonin. E.V., Tian, T. & Falk, B.W. 1995. Genome structure and phylogenetic analysis of lettuce infectious yellows virus, a whitefly-transmitted closterovirus.  Virology 208: 99-110.
  18. Tian, T. , Medina, V., Mayhew, D. E., Maeda, S., and Falk, B. W. 1995. Beet western yellows luteovirus capsid proteins produced by recombinant baculoviruses assembles into virion-like particles in cells and larvae of Bombyx mori.  Virology 213:204-212.